Here, we provide a thorough protocol for the dissection, extraction, and acute horizontal slicing of the murine brain, and discuss challenges and potential pitfalls of this technique. Like the cerebral cortex, with which it is closely associated, it is a paired structure, with mirror-image halves in the left and right sides of the brain. Temporal dynamics of mouse hippocampal clock gene expression support memory processing Antje Jilg Institute of Cellular and Molecular Anatomy, Dr. It belongs to the limbic system and plays important roles in long-term memory and spatial navigation. In 2011, the reference atlas was updated to enable interactive online exploration of the atlas and to provide a deeper level of 3-D annotation for informatics. The hippocampus is a major component of the brains of humans and other mammals. In contrast to other slice preparations, horizontal slicing allows to keep the fibers of the hippocampal input path (perforant path) in a fully intact state within a slice, which facilitates the investigation of entorhinal-hippocampal interactions. The Allen Mouse Brain Atlas includes a full-color, high-resolution anatomic reference atlas accompanied by a systematic, hierarchically organized taxonomy of mouse brain structures. Here, a detailed protocol for a successful preparation of vibratome-cut horizontal hippocampal slices of mouse brains is provided. Moreover, different cutting planes can be applied for vibratome brain slice preparations. Brain slice preparations with a vibratome allow a better preservation of the tissue structure and guarantee a sufficient oxygen supply during slicing, which present advantages over the traditional use of a tissue chopper. There exist several different ways of slice preparations. Therefore, brain slices represent an ideal model system to assess protein functions, to investigate pathophysiological processes involved in neurological disorders as well as for drug discovery purposes. Hippocampal brain slices can be used for several different applications, including electrophysiological recordings, light microscopic measurements as well as several molecular biological and histochemical techniques. Brain slices are a frequently used methodology when exploring neurophysiological functions of the hippocampus. This connectivity is accomplished via different informational flows in the form of fiber pathways. infrapyramidal) in adult neurogenesis in the mouse hippocampus using endogenous markers.
Here, we examined the topographic differences (dorsal vs. The hippocampus receives a high degree of intra- and inter-connectivity, securing a proper communication with internal and external brain structures. For instance, dorsal hippocampus is involved in spatial memory and learning whereas ventral hippocampus is related to emotion. The hippocampus is a highly organized structure in the brain that is a part of the limbic system and is involved in memory formation and consolidation as well as the manifestation of severe brain disorders, including Alzheimer's disease and epilepsy.
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